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分别给非洲爪蟾卵母细胞微量注射电线电器官烟碱型胆碱受体（Ｎ＿２－ＣｈＲ）和大鼠脑烟碱型胆碱受体（Ｎ＿１－ＣｈＲ）的ｍＲＮＡ，表达出相应的Ｎ－ＣｈＲ。采用全细胞和部分细胞灌流法测定不同浓度氨甲酰胆碱引起的Ｎ＿２－ＣｈＲ产生的膜电流，以最小反应模型方程拟合两法所得量反应曲线非常接近。Ｋ＿１值分别为０．５２８和０．４９１ｍｍｏｌ·Ｌ￣（－１）；Φ￣（－１）值分别为３．８６１和３．８９１。采用全细胞灌流法测得不同浓度氨甲酰胆碱引起的表达Ｎ＿１－ＣｈＲ产生的膜电流，拟合所得的量反应曲线较之相应的Ｎ２－ＣｈＲ曲线明显左移。Ｋ＿１和Φ￣（－１）值分别为０．１４０ｍｍｏｌ·Ｌ￣（－１）和３．１２５。
The mRNAs of nicotinic cholinergic receptor (N 2 -chR) and nicotinic acetylcholine receptor (N 1 -chR) in the electrical organ of Xenopus laevis were microinjected into the oocytes of Xenopus laevis oocytes, respectively, and the corresponding N- ChR. Whole cell and partial cell perfusion method was used to determine the concentration of carbachol-induced N 2 -chR membrane current, and the response curve fitted by the two reaction model equations was very close. The values of K_1 were 0.528 and 0.491 mmol·L -1, respectively. The values of Φ ~ (-1) were 3.861 and 3.891, respectively. Whole cell perfusion method was used to measure the concentration of carbachol induced expression of N 1 -chR membrane current, the resulting volume response curve than the corresponding N2-ChR curve shifted to the left. K_1 and Φ ~ (-1) values were 0.140mmol·L ~ (-1) and 3.125 respectively.