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目的:观察安坤种子丸对胚泡着床障碍小鼠雌、孕激素受体表达的影响,探讨其对胚泡着床障碍小鼠子宫内膜容受性的影响及作用机制。方法:筛选有规律动情周期的雌性小鼠,随机分为正常组、模型组和中药组,每组25只。用安坤种子丸先期对中药组小鼠进行灌胃,雌、雄合笼后观察雌鼠阴栓情况,之后各组选取有阴栓者20只并计为妊娠第1天(D1),雄鼠及其余雌鼠刎除。妊娠第4天对中药组和模型组注射米非司酮进行干预,制成胚泡着床障碍模型;于妊娠第5、6天处死小鼠,留取子宫标本。检测子宫雌激素受体(ER)、孕激素受体(PR)的表达;同时统计分析各组间妊娠率、平均着床胚泡数及胚泡着床率等指标的变化。结果:安坤种子丸可以提高胚泡着床障碍小鼠子宫内膜ER、PR的表达,妊娠率、平均着床胚泡数及胚泡着床率等指标虽低于正常组(P<0.05),但显著高于模型组(P<0.01)。结论:安坤种子丸可以提高雌鼠子宫内膜上ER、PR的水平,一定程度上改善着床障碍小鼠子宫内膜容受性,促进胚泡着床。
Objective: To observe the effect of Ankun seed pill on the expression of estrogen and progesterone receptor in blastocyst implantation dysfunction mice, and to explore its effect on endometrial receptivity in mice with blastocyst implantation dysfunction and its mechanism. Methods: Female mice with regular estrous cycle were screened and randomly divided into normal group, model group and traditional Chinese medicine group, with 25 rats in each group. Ankun seed pills were used to treat the mice of traditional Chinese medicine group by gavage. Female and male were caged to observe the female thrombus. After that, 20 rabbits in each group were selected and counted as the first day of gestation (D1) and male Mice and other females removed. On the 4th day of gestation, the mifepristone was injected into the Chinese medicine group and the model group to make the embryo implantation dysfunction model. The mice were sacrificed on the 5th and 5th day of gestation, and the uterus specimens were collected. The expressions of estrogen receptor (ER) and progesterone receptor (PR) in uterus were detected. The changes of pregnancy rate, average number of implanted blastocysts and blastocyst implantation rate were also statistically analyzed. Results: Ankun seed pills could improve the expression of ER and PR in endometrium of blastocyst implantation dysfunction mice. The pregnancy rate, average number of blastocyst and blastocyst implantation rate were lower than those in normal group (P <0.05 ), But significantly higher than the model group (P <0.01). Conclusion: Ankun seed pills can increase the levels of ER and PR in female endometrium, and improve the endometrial receptivity of impaired mice in some extent and promote the implantation of blastocysts.