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目的：重组表达人ＡＣＡＴ的Ｎ端片段。方法：将编码ＡＣＡＴ氨基末端包含第一个跨膜区（１～１９６ａａ）的ｃＤＮＡ片段克隆至不同的大肠杆菌表达载体中，构建了多种大肠杆菌表达质粒，再转化至大肠杆菌中进行表达研究。结果和结论：在详细摸索表达条件的基础上，经温敏诱导，成功地在大肠杆菌ＡＲ６８中表达了Ｎ端融合ｐｒｏｔｅｉｎＡＢＣｄｏｍａｉｎ的人ＡＣＡＴ氨基末端包含第一个跨膜区的片段，表达量约２０ｍｇ／Ｌ菌液。为进一步表达完整的ＡＣＡＴ蛋白、研究ＡＣＡＴ基因表达调控及其结构与功能的关系奠定了基础
Objective: Recombinant expression of human ACAT N-terminal fragment. Methods: The cDNA fragment containing the first transmembrane region (1 ~ 196aa) encoding the amino terminal of ACAT was cloned into different E.coli expression vectors. A number of E.coli expression plasmids were constructed and transformed into E.coli for expression study . RESULTS AND CONCLUSION: Based on the detailed exploration of the expression conditions, the amino terminal of the human ACAT with N-terminal fusion proteinABC domain successfully expressed in Escherichia coli AR68 was induced by temperature-sensitive and contained the first transmembrane region. The expression level was about 20mg / L bacteria solution. In order to further express the complete ACAT protein, the study of ACAT gene expression regulation and its relationship with the structure and function laid the foundation