论文部分内容阅读
为建立水稻耐冷育种的新途径，开展了本项试验研究。结果是：①在１５℃下愈伤组织净生长量与供体品种孕穗期的耐冷性呈显著的正相关，其中２５天的相关系数最大。②１５℃下选择的愈伤组织的无性系中６２．５％的无性系耐冷性明显强于供体品种。③体细胞无性系２、３代的广义遗传力高。④供体品种孕穗期的耐冷性与耐冷变异体率呈极显著的负相关，与感冷变异体率呈正相关。根据上述试验结果，认为１５℃冷温作胁迫条件，愈伤组织生长量作形态指标，２５天作一个选择周期反复选择多次耐冷愈伤组织系的方法，作为粳稻体细胞耐冷变异体的诱导筛选技术
In order to establish a new way of cold-tolerant rice breeding, this pilot study was carried out. The results were as follows: ① There was a significant positive correlation between net growth of callus and cold tolerance at donor booting stage at 15 ℃, of which the correlation coefficient was the highest at 25 days. ② The cold tolerance of 62.5% clones in the selected callus clones at 15 ℃ was significantly stronger than that of the donor varieties. ③ somatic clones 2,3 generations of high heritability. ④ The cold tolerance of donor varieties at booting stage was significantly negatively correlated with the rate of cold tolerant variants, which was positively correlated with the rate of cold variant. According to the above test results, it is considered that cold stress at 15 ℃ and morphological index of callus growth, and selection of multiple cold-tolerant callus lines at a selective cycle for 25 days are selected as inducing screening of somatic cold tolerance mutant of japonica rice technology