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目的研究2,2,6,6-四甲基-3-烯哌啶氮氧自由基鱼藤酮肟酯(PNR)体外抗肿瘤作用。方法用磺酰罗丹明B(SRB)法检测PNR对肿瘤细胞增殖能力的影响;用流式细胞术检测PNR对Tca8113细胞周期的影响;用Transwell迁移法观察PNR对Tca8113细胞迁移能力的影响;用4’,6-二脒基-2-苯基吲哚(DAPI)荧光染色法观察Tca8113细胞凋亡形态。结果 PNR对Tca8113、A549、Hep G 2、BCG 823和EJ等人癌细胞均有增殖抑制作用。PNR在0.8~12.8μg/m L剂量范围内浓度、时间依赖性地抑制A549、Hep G 2和BCG 823细胞的生长。2~7μg/m L明显抑制Tca8113和EJ细胞生长,量效关系明显,对Tca8113和EJ细胞抑制作用在24 h已达高峰。综合比较,PNR对Tca8113细胞的抑制作用最强。PNR还明显抑制Tca8113细胞的迁移活性,阻止Tca8113细胞于S期,减少G1期细胞比例,并能明显诱导Tca8113细胞凋亡。结论 PNR对多种肿瘤细胞的生长有明显抑制作用,对Tca8113细胞的作用最强,并能抑制其迁移,诱导其凋亡。
Objective To study the antitumor effect of 2,2,6,6-tetramethyl-3-enepiperidine nitroxide rotenone oxime ester (PNR) in vitro. Methods The effect of PNR on the proliferation of tumor cells was detected by the method of SRB. The effect of PNR on the cell cycle of Tca8113 was detected by flow cytometry. The effect of PNR on the migration of Tca8113 cells was observed by Transwell migration assay. 4 ’, 6-diamidino-2-phenylindole (DAPI) staining was used to observe the apoptotic morphology of Tca8113 cells. Results PNR inhibited the proliferation of cancer cells such as Tca8113, A549, Hep G2, BCG 823 and EJ. PNR concentration in the dose range of 0.8 ~ 12.8μg / m L, in a time-dependent manner inhibited the growth of A549, Hep G2 and BCG 823 cells. 2 ~ 7μg / m L significantly inhibited the growth of Tca8113 and EJ cells, the dose-response relationship was obvious, and the inhibitory effect on Tca8113 and EJ cells reached the peak at 24 h. In summary, PNR had the strongest inhibitory effect on Tca8113 cells. PNR also significantly inhibited the migration of Tca8113 cells, prevented Tca8113 cells from S phase, decreased the proportion of cells in G1 phase and significantly induced the apoptosis of Tca8113 cells. Conclusions PNR can significantly inhibit the growth of many tumor cells and has the strongest effect on Tca8113 cells, and can inhibit its migration and induce its apoptosis.