S-wave velocity structure beneath the Ailaoshan-Red River fault was obtained from receiver functions by using teleseismic body wave records of broadband digital seismic stations. The average crustal thickness, Vp/Vs ratio and Poisson’s ratio were also estimated. The results indicate that the interface of crust and mantle beneath the Ailaoshan-Red River fault is not a sharp velocity discontinuity but a characteristic transition zone. The velocity increases relatively fast at the depth of Moho and then increases slowly in the uppermost mantle. The average crustal thickness across the fault is 36―37 km on the southwest side and 40―42 km on the northeast side, indicating that the fault cuts the crust. The relatively high Poisson’s ratio (0.26―0.28) of the crust implies a high content of mafic materials in the lower crust. Moreover, the lower crust with low velocity could be an ideal position for decoupling between the crust and upper mantle. The average crustal thickness, Vp / Vs ratio and Poisson’s ratio were also estimated. The results indicate that the interface of crust and mantle beneath the Ailaoshan-Red River fault is not a sharp velocity discontinuity but a characteristic transition zone. The velocity increases relatively the depth of Moho and then increase slowly in the uppermost mantle. The average crustal thickness across the fault is 36-37 km on the southwest side and 40-42 km on the northeast side, indicating that the fault cuts the crust. The relatively high Poisson’s ratio (0.26-0.28) of the crust implies a high content of mafic materials in the lower crust. Moreover, the lower crust with low velocity could be an ideal position for decoupling between the crust and upper mantle.