临床医学发现,给病人服用四环素后定时测定体液中四环素含量,可以诊断胃癌~[1],因此.建立灵敏、准确的四环素测定方法具有重要意义.已知四环素类抗菌素与一些金属离子有很强的螯合能力,并能生成有色螯合物,其中以锆、钍、铀、锌、铜、铝、镁、铈及钴的螯合物尤为稳定,可用于鉴别、测定四环素类抗菌素的含量.一些螯合物也用于荧光测定~[2～6],但 Clinical medicine found that tetracycline patients taking tetracycline after the regular determination of body fluid tetracycline content, can diagnose gastric cancer ~ [1] Therefore, the establishment of sensitive and accurate determination of tetracycline is of great significance.It is known tetracycline antibiotics and some metal ions have a strong Chelating ability and can generate colored chelates, among which zirconium, thorium, uranium, zinc, copper, aluminum, magnesium, cerium and cobalt chelates are particularly stable and can be used for identification and determination of the content of tetracycline antibiotics. Some chelates are also used for fluorescence determination ~ [2 ~ 6], but