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目的探讨利用缓释泵法模拟多因素诱导急性胰腺炎(AP)大鼠模型的相关技术。方法将75只健康SD大鼠按随机表法随机均分为缓释泵组(SRP组)、传统组(TAP组)和假手术组(SO组),TAP组采用胆胰管逆行注射法,SRP组应用缓释泵注射4%牛磺胆酸钠,从自我消化、梗阻、细胞因子激活等多方面机理模拟AP发病机理;3组大鼠均在模型诱导后1、6、12及24 h后检测血清淀粉酶及胰腺组织髓过氧化物酶(MPO)水平,观察胰腺组织病理学改变,并进行胰腺病变程度评分。结果 在模型诱导后1、6、12及24 h,SRP组和TAP组大鼠的血清淀粉酶及MPO水平和胰腺病变程度评分均明显高于SO组(P<0.05或P<0.01),而SRP组各时点的胰腺病变程度评分以及血清淀粉酶和胰腺组织中MPO水平升高程度均较TAP组低,上升趋势相对较缓,其中6和12 h的差异有统计学意义(P<0.05或P<0.01)。结论 缓释泵法可成功诱导AP,该方法可从增加胰管内压、导致组织水肿、持续释放炎症刺激因子等多层面模拟胰腺炎病理生理过程,通过留置泵可进一步监测、控制胰管内压或用于进一步治疗。缓释泵法作用相对较缓,造模过程相对容易管理,死亡率较低。
Objective To explore the use of slow release pump method to simulate the multi-factor induced acute pancreatitis (AP) rat model. Methods Seventy-five healthy SD rats were randomized into SRP group (SRP group), TAP group (sham group) and sham operation group (SO group) according to random table method. TAP group was treated by retrograde cholangiopancreatography SRP group was injected with 4% sodium taurocholate by slow-release pump, and the pathogenesis of AP was simulated from many aspects such as self-digestion, obstruction and activation of cytokines. The rats in the three groups were exposed to 1, 6, 12 and 24 h after model induction Serum amylase and pancreatic myeloperoxidase (MPO) levels were measured to observe the histopathological changes in the pancreas, and the degree of pancreatic lesions were scored. Results At 1, 6, 12 and 24 h after model induction, serum amylase, MPO and pancreatic lesions in SRP group and TAP group were significantly higher than those in SO group (P <0.05 or P <0.01), respectively The severity of pancreatic lesions at each time point in SRP group and MPO level in serum amylase and pancreatic tissue were lower than those in TAP group, and the upward trend was relatively slow, with a significant difference between 6 and 12 h (P <0.05 Or P <0.01). Conclusion The slow-release pump method can induce AP successfully. This method can simulate the pathophysiological process of pancreatitis by increasing the internal pressure of the pancreatic duct, leading to tissue edema and sustained release of inflammatory stimuli. The left ventricular pump can further monitor and control the internal pressure of the pancreatic duct or For further treatment. Slow release pump method is relatively slow, the modeling process is relatively easy to manage, the mortality rate is low.