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以100份茄子种质资源中筛选出的耐低温基因型材料E659和低温敏感基因型材料E646为试材,研究了低温处理对不同耐寒性茄子叶片的光抑制能力、丙二醛含量、电导率、过氧化氢酶(CAT)、抗坏血酸酶(APX)和超氧化物歧化酶(SOD)活性的影响。结果表明,在4℃处理5 d后,与低温敏感材料E646相比,低温胁迫下筛选获得的极耐低温基因型材料E659,F V/F M比值下降较少,表明其PSII系统受损较少,而丙二醛含量和电导率上升幅度较低则表明膜脂过氧化反应引起的氧化损伤较轻。4℃低温处理24h之内,发现E659植株中抗坏血酸酶活性增高,过氧化氢酶和超氧化物歧化酶的活性损害减弱或时间推迟。可见,茄子耐低温基因型材料E659在低温胁迫下能快速启动应激反应,增强氧化系统清除能力,减轻低温对植株生长发育的伤害。
A total of 100 eggplant germplasm resources selected from the low temperature resistant genotype material E659 and low temperature sensitive genotype material E646 were used as materials to study the effects of low temperature treatment on photosynthetic ability, MDA content, electrical conductivity , Catalase (CAT), ascorbate (APX) and superoxide dismutase (SOD) activity. The results showed that compared with E646, the E659 and FV / FM ratios of E659, which were obtained by low temperature stress screening, decreased less after treatment at 4 ℃ for 5 d, indicating that their PSII system had less damage, However, the lower MDA content and conductivity increase indicates that the oxidative damage caused by membrane lipid peroxidation is lighter. Under low temperature treatment at 4 ℃ for 24h, it was found that ascorbate activity in E659 plants was increased, and the activity of catalase and superoxide dismutase was weakened or delayed. Thus, eggplant low temperature resistant genotype E659 can quickly start stress response under low temperature stress, enhance the scavenging capacity of the oxidative system and reduce the damage of low temperature on plant growth and development.