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基因蛋白质(酶)的合成、生物体间酶系统的差异在一定程度上反映了生物体之间遗传基础的差异。过氧化物酶广泛存在于植物体内,受不同结构基因的控制构成了过氧化物酶同工酶酶谱的多样性。根据过氧化物酶同工酶酶谱的差异可以判断玉米杂交种或自交系间遗传基础的相对差异。利用同工酶或电泳技术在水稻、小麦、玉米等农作物品种鉴别中的利用国内外已有报道,可是对于酶谱的描述则尽限于利用图谱、泳动率和仪器扫描图的分析,前者简便易行但不能显示出体现酶活性的酶带宽度。后者灵敏度高但存在着难以描述和根据这一指标恢复酶谱原形的问题。本研究的目的是通过过氧化物酶同工酶的酶谱编码达到方便酶谱比较和描述的目的。
Gene protein (enzyme) synthesis, differences in the enzyme system between organisms to some extent, reflects the genetic basis of the differences between organisms. Peroxidase is widely present in plants, which is controlled by different structural genes and constitutes a diversity of peroxidase isozymes. Based on the differences in peroxidase isoenzyme patterns, the relative differences in genetic basis between maize hybrids or inbred lines can be judged. The use of isoenzyme or electrophoresis in the identification of rice, wheat, maize and other crops has been reported at home and abroad, but for the description of the enzyme is limited to the use of maps, mobility and instrument scan analysis, the former is simple Easy but does not show the enzyme activity of the enzyme band width. The latter is highly sensitive but has the problem of being difficult to describe and restoring the original shape of the zymogram based on this index. The purpose of this study is to achieve the purpose of comparison and description of the enzyme zymogram through the zymogram coding of peroxidase isozymes.