论文部分内容阅读
目的:建立快速测定L-谷氨酰胺呱仑酸钠颗粒中有效成分含量的离子迁移谱方法。方法:采用离子迁移谱正模式测定L-谷氨酰胺含量,电离源电压3 k V;呱仑酸钠测定时为负模式,电离源电压2.6 k V;迁移管电压均为8 k V,电离源和迁移管温度均为180℃。结果:L-谷氨酰胺和呱仑酸钠质量浓度均在10~200μg·m L-1范围内与峰面积线性关系良好,相关系数分别为0.998 2和0.997 4;定量限分别为10μg·m L-1和6μg·m L-1;L-谷氨酰和呱仑酸钠胺高、中、低3个浓度平均回收率均在97%~103%之间。每批样品连续测定6次,L-谷氨酰胺的平均含量分别为100.4%、101.0%和98.8%,RSD分别为2.2%、2.2%和2.3%;呱仑酸钠的平均含量分别为100.7%、101.7%和99.1%,RSD分别为2.7%、2.3%和2.7%。结论:离子迁移法适用于L-谷氨酰胺呱仑酸钠颗粒中有效成分含量测定。
OBJECTIVE: To establish an ion mobility spectrometry method for the rapid determination of active ingredients in L-glutamine sodium particles. Methods: The content of L-glutamine was determined by ion mobility spectrometry positive mode with an ionization voltage of 3 kV. The sodium gallic acid assay was negative mode with an ionization voltage of 2.6 kV. The voltage of the transfer tube was 8 kV and ionization Source and transfer tube temperature is 180 ℃. Results: The linear ranges of L-glutamine and sodium goronate in the range of 10-200 μg · m L-1 were linear with the peak area, the correlation coefficients were 0.998 2 and 0.997 4, respectively. The limits of quantification were 10 μg · m L-1 and 6μg · m L-1. The average recoveries of L-glutamyl and sodium valproate at high, middle and low concentrations were between 97% and 103%. The average contents of L-glutamine were 100.4%, 101.0% and 98.8%, respectively, and the RSDs were 2.2%, 2.2% and 2.3% respectively. The average contents of sodium galenic acid were 100.7% , 101.7% and 99.1% with RSDs of 2.7%, 2.3% and 2.7% respectively. Conclusion: The ion mobility method is suitable for the determination of the active ingredients in L-glutamine sodium gorinate particles.