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血管内皮细胞在维持血管壁的完整性、调节微血管通透性和血液的流动性等方面具有重要作用。内皮细胞不仅有较强的抗血栓作用,参与血栓的溶解过程,而且具有重要的代谢功能:如灭活某些血管活性物质、摄取脂蛋白等。因而内皮细胞损伤可能是血栓形成、动脉粥样硬化及脏器、组织水肿的重要原因。为研究某些病理因素对内皮细胞的影响,探讨肺水肿等病征的发生机制,我们参照Jaffe等人的方法,建立了血管内皮细胞培养法,现介绍如下:
Vascular endothelial cells play an important role in maintaining the integrity of the vascular wall, regulating microvascular permeability and blood fluidity. Endothelial cells not only have a strong anti-thrombotic effect, involved in the dissolution process of thrombus, but also have important metabolic functions: such as inactivation of certain vasoactive substances, lipoproteins and so on. Thus endothelial cell damage may be an important cause of thrombosis, atherosclerosis and organs, tissue edema. In order to study the effect of certain pathological factors on endothelial cells and explore the mechanism of the pathogenesis such as pulmonary edema, we established the method of vascular endothelial cell culture according to the method of Jaffe et al.