论文部分内容阅读
目的:研究东北林蛙(Rana dybowskii Günther,1876)皮肤抗菌肽(dybowskin-1ST)对小鼠皮肤损伤模型创伤愈合的影响。方法:将60只健康成年小鼠随机分为甘油对照组、外用应急软膏组和dybowskin-1ST组(50 mg.L-1,10μg/只),制备皮肤损伤模型,将供试品按0.2 mL/只的剂量涂抹创口外延至2～3 mm,1次/d。于损伤后第1,3,5,7天测量皮肤损伤面积,计算伤口愈合率,第3,7天取损伤部位皮肤,组织切片观察其形态学变化。结果:第7天dybowskin-1ST组和外用应急软膏组小鼠皮肤损伤部位愈合率分别为(86.74±0.27)%和(91.57±0.14)%,与甘油对照组比较,提高了16.04%和20.87%,P<0.05;组织形态学显示,dybowskin-1ST组愈合初期新生组织的毛细血管和成纤维细胞数量增多,愈合后期组织分层明显,且与外用应急软膏组无明显差异。结论:dybowskin-1ST能改善小鼠损伤部位肉芽组织生长状态和胶原纤维形成,促进伤口愈合。
Objective: To study the effect of skin antimicrobial peptide (dybowskin-1ST) from Rana dybowskii Günther (1876) on wound healing in mouse skin injury model. Methods: 60 healthy adult mice were randomly divided into glycerol control group, topical emergency ointment group and dybowskin-1ST group (50 mg.L-1,10μg / only) to prepare skin damage model. / Only dose smear wound extension to 2 ~ 3 mm, 1 time / d. On the 1st, 3rd, 5th and 7th day after injury, the area of skin injury was measured. The rate of wound healing was calculated. On the 3rd and 7th days, the skin of injured area was taken and the histological changes were observed. Results: The healing rates of skin lesions in dybowskin-1ST group and topical emergency ointment group on day 7 were (86.74 ± 0.27)% and (91.57 ± 0.14)%, respectively, increased by 16.04% and 20.87% compared with the glycerol control group , P <0.05. Histomorphology showed that the number of capillaries and fibroblasts in dybowskin-1ST group increased at the early healing stage, and the tissue layers at the later stage of hemorrhage were obviously stratified. There was no significant difference between the group and the topical emergency ointment group. Conclusion: dybowskin-1ST can improve the growth of granulation tissue and the formation of collagen fibers in wound site and promote wound healing.