Bacteriostatic laundry detergent was prepared with application of dodecyl dimethyl benzyl ammonium chloride (1227).In accordance with relevant standards applied in Chinese detergent industry,the effect of 1227 on sensory and physiochemical quality indices,detergency and foaming performance of laundry detergent were comprehensively investigated,and antimicrobial performance as well as practical application performance including skin irritation of laundry detergent formulated with 1227 was emphatically examined and compared with that of market product with similar functions.Results indicated that,in formulation without anionic surfactant,all indices of self-prepared bacteriostatic laundry detergent can meet requirements specified in relevant standards.The foaming power of self-prepared bacteriostatic laundry detergent was lower and easier to rinse than bacteriostatic laundry detergent product in market.Using aqueous solution of 1％ mass fraction of self-prepared bacteriostatic laundry detergent containing 2％ of 1227,the antibacterial rate for 20 min on both Escherichia coli and Staphylococcus aureus separately reached 100％,which was much better than that of the bacteriostatic laundry detergent product from market.The action effectiveness of the self-prepared bacteriostatic laundry detergent can be maintained for a longer time period and shows no obvious irritation effect to human skin.