论文部分内容阅读
绿藻素是营养全面的保健食品。ＶＢ_５在绿藻素中的含量丰富。本法以 ０．２５ｍｏｌ／ＬＨ_２ＳＯ_４： ９５％乙醇＝１＋１为 提取剂，流动相为甲醇：异丙醇：十二烷基磺酸钠（０．５ｇ／Ｌ）＝７＋２＋１，并用Ｈ_３ＰＯ_４调节ｐＨ＝２．５，在Ｃ_１８反相柱上 得到了良好的分离。其回收率为９６．０％，相对标准偏差为２．９２％，标准差为０．００５８１，样品提取液经０．４５μｍ滤膜过滤 后可直接上机进样。本法为保健食品绿藻素中的ＶＢ_５的测定提供了可行的检测分析方法。
Chlorella is a comprehensive nutrition health food. VB_5 is rich in chlorophyll. In this method, methanol: isopropanol: sodium dodecyl sulfate (0.5g / L) = 7 + 2 + 1 was used as mobile phase with 0.25mol / LH 2 SO 4: 95% ethanol = 1 + 1 as the mobile phase and the pH was adjusted to 2 with H_3PO_4 .5, a good separation on the C_ 18 reverse phase column. The recovery was 96.0%, the relative standard deviation was 2.92%, and the standard deviation was 0.00581. The sample extract was filtered on a 0.45μm filter and then directly on the machine. This method provides a feasible detection and analysis method for the determination of VB_5 in health food chlorophyll.