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基于表面等离子体共振(Surface plasmon resonance,SPR)原理的基因芯片检测方法不需要标记,是一种很有潜力的高通微量分析(High-throughput microanalysis,HTMA)方法。应用自组装单分子层技术(Self-as-sembled monolayer,SAM)制备淋病奈瑟菌探针点阵的基因芯片,应用自行组建的SPR和表面等离子体共振成像(SPR imaging,SPRI)基因芯片分析系统,对该基因芯片进行检测分析,研究基因芯片上探针点阵的杂交反应。结果表明:SPR共振曲线上都有明显的共振吸收峰,探针杂交后折射率增大,共振角增大,分子量增大。由SPR检测界面或共振曲线可判断待分析样本与基因芯片上的探针是否发生杂交反应,待分析样本中是否含有待检测的物质。由SPRI检测系统可实时、直观地观察基因芯片上的探针是否发生杂交反应。SPR和SPRI系统可进行定性和定量分析。
Gene chip detection based on surface plasmon resonance (SPR) principle does not require labeling and is a potential high-throughput microanalysis (HTMA) method. The gene chip of Neisseria gonorrhoeae probe array was prepared by using self-as-sembled monolayer (SAM). SPR imaging and SPRI imaging System, the detection of the gene chip analysis, gene chip probe lattice hybridization reaction. The results showed that there were obvious resonance absorption peaks on the resonance curve of SPR. After the hybridization, the refractive index increased, the resonance angle increased and the molecular weight increased. SPR detection interface or resonance curve can be judged by the sample to be analyzed with the gene chip hybridization reaction probe is to be analyzed whether the sample contains the substance to be detected. By SPRI detection system can real-time and intuitive observation of gene chip hybridization probe. The SPR and SPRI systems allow qualitative and quantitative analysis.