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目的探讨阿霉素诱导人涎腺癌细胞凋亡的分子机制。方法通过光镜和电镜观察不同作用时间后细胞的形态学改变，ＤＮＡ琼脂糖凝胶电泳，流式细胞仪分析ＤＮＡ含量和细胞周期，并检测细胞内Ｃａ(２＋)浓度的变化。结果阿霉素作用于ｍＥｃ－１细胞后出现了细胞凋亡的特征，细胞内Ｃａ(２＋)浓度明显增力。。结论Ｃａ(２＋)参与细胞凋亡过程可能是阿霉素诱导ＩＥＣ－１细胞凋亡的机制之一。
Objective To investigate the molecular mechanism of doxorubicin-induced apoptosis of human salivary gland cancer cells. Methods The morphological changes of cells were observed by light microscope and electron microscope. DNA agarose gel electrophoresis, DNA content and cell cycle were analyzed by flow cytometry, and the change of intracellular Ca(2+) concentration was detected. RESULTS: After doxorubicin was applied to mEc-1 cells, there was a characteristic of apoptosis, and the concentration of intracellular Ca(2+) increased significantly. . Conclusion The involvement of Ca(2+) in apoptosis may be one of the mechanisms of doxorubicin-induced apoptosis of IEC-1 cells.