本实验用ESR测定岛状皮瓣在缺血/再灌流后自由基总量的变化和用TBA法测定了皮瓣组织再灌流后MDA的变化。结果表明岛状皮瓣在缺血/再灌流之后,氧自由基含量明显增加,脂质过氧化物含量也明显增高,并且两者间呈直线正相关。结果提示,氧自由基是造成岛状皮瓣再灌流损伤的主要病理因素,而造成损伤的直接原因则是氧自由基增加后所引起的脂质过氧化反应。 In this study, the total amount of free radicals in the island flap after ischemia / reperfusion was measured by ESR and the MDA content in the flap tissue after reperfusion was measured by TBA method. The results showed that after the ischemia / reperfusion, the island flap significantly increased the content of oxygen free radicals and the content of lipid peroxides, and there was a linear positive correlation between the two. The results suggest that oxygen free radicals are the main pathological factors leading to reperfusion injury of island flaps, and the direct cause of injury is the lipid peroxidation caused by the increase of oxygen free radicals.