论文部分内容阅读
目的:探讨兔急性肺血栓栓塞症时脑尿钠肽(BNP),血清炎症因子在肺栓塞及溶栓时的变化情况及血必净注射液对它们的影响。方法:选用30只中国大耳白兔,随机分为对照组、模型组、单纯溶栓(尿激酶)组(UK组),血必净注射液+尿激酶溶栓治疗组(UK加血必净注射液组),单纯血必净注射液治疗组,每组6只。采用兔自体血栓回输法建立急性肺栓塞模型。采用干式快速免疫荧光法检测血清BNP的变化,采用酶联免疫吸附法测定肿瘤坏死因子-α(TNF-ɑ),白细胞介素-8(IL-8)含量的变化。观察各组生命体征变化。结果:栓塞后,与对照组相比,其他组BNP,TNF-α升高(P<0.05)。溶栓后,与模型组相比,UK组BNP含量降低,而TNF-ɑ、IL-8升高(P<0.05);单纯血必净注射液组TNF-α,IL-8下降明显(P<0.05),BNP轻微下降(P>0.05)。UK加血必净注射液组3者含量全部显著下降(P<0.01)。结论:血必净注射液对急性肺栓塞溶栓的影响主要是通过抑制内皮细胞的炎性反应而起保护作用。
Objective: To investigate the changes of brain natriuretic peptide (BNP) and serum inflammatory cytokines in pulmonary embolism and thrombolysis in rabbits with acute pulmonary thromboembolism and the effect of Xuebijing injection on them. Methods: 30 Chinese white rabbits were randomly divided into control group, model group, simple thrombolysis (UK group), Xuebijing injection + Urokinase thrombolysis group Net injection group), pure Xuebijing injection treatment group, 6 in each group. Acute pulmonary embolism model was established by rabbit autologous thrombosis. Serum BNP levels were detected by dry-fast immunofluorescence. The levels of tumor necrosis factor-α (TNF-ɑ) and interleukin-8 (IL-8) were measured by enzyme-linked immunosorbent assay. The changes of vital signs in each group were observed. Results: After embolization, the levels of BNP and TNF-α in other groups were significantly higher than those in control group (P <0.05). After thrombolytic therapy, BNP levels in UK group decreased and TNF-ɑ and IL-8 levels increased (P <0.05) compared with model group; TNF-α and IL-8 decreased significantly in Xuebijing Injection group <0.05), BNP decreased slightly (P> 0.05). UK plus Xuebijing injection group 3 all decreased significantly (P <0.01). Conclusion: The effect of Xuebijing Injection on thrombolysis in acute pulmonary embolism is mainly through inhibiting the inflammatory reaction of endothelial cells.