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A new fluorescence sensor is developed for simultaneous detection of Hg~(2+) and Ag~+. During the detection process,Hg~(2+) forms complexes with the fluorescent dye rhodamine B isothiocyanate(RBITC) modified onto the surface of gold nanoparticles(Au NPs),resulting in RBITC’s displacement from the surface of Au NPs and the recovery of fluorescence. Meanwhile,Ag~+ forms “C-Ag~+-C” complex with C-rich 5-carboxyfluorescein(FAM)-ss DNA modified onto the surface of Au NPs,which keeps the fluorescent dye FAM close to the Au NPs and results in quench of fluorescence. The experimental results show a wide linear range and a good sensitivity. The limit of detection is 1.06 nmol/L for Ag~+ and 0.48 nmol/L for Hg~(2+). This detection method is not only easy to operate but also efficient.
During the detection process, Hg 2+ forms complexes with the fluorescent dye rhodamine B isothiocyanate (RBITC) modified onto the surface of gold nanoparticles (Au NPs), resulting in RBITC’s displacement from the surface of Au NPs and the recovery of fluorescence. Meanwhile, Ag ~ + forms “C-Ag ~ + -C” complex with C-rich 5-carboxyfluorescein The ssDNA modified onto the surface of Au NPs, which keeps the fluorescent dye FAM close to the Au NPs and results in quench of fluorescence. The experimental results show a wide linear range and a good sensitivity. The limit of detection is 1.06 nmol / L for Ag ~ + and 0.48 nmol / L for Hg ~ (2+). This detection method is not only easy to operate but also efficient.