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目的:探讨趋化因子CXCR4在甲状腺乳头状癌中的表达及其与临床病理学参数的相关性。方法:利用半定量RT-PCR和免疫组织化学法分别检测72例新鲜甲状腺乳头状癌组织及52例甲状腺乳头状癌石蜡标本中CXCR4mRNA及蛋白的表达情况。结果:甲状腺乳头状癌组织中CXCR4mRNA及蛋白的阳性表达率分别为77.8%(56/72)、76.6%(95/124),而甲状腺良性病变组织及正常甲状腺组织均无表达,差异具有统计学意义(P<0.01);在甲状腺乳头状癌组织中,CXCR4mRNA表达与蛋白表达成正相关关系(r=0.714,P<0.01)。单因素分析显示CXCR4的蛋白表达与年龄、性别、肿瘤大小无关(P>0.05),但与肿瘤侵犯程度、淋巴结转移、远处转移、临床分期密切相关(P<0.05),且CXCR4蛋白阳性表达组的预后显著差于CXCR4蛋白阳性表达组(P<0.05)。结论:CXCR4阳性表达的甲状腺乳头状癌具有较高侵袭转移潜能,是预后不良的指标之一,CXCR4可作为抑制甲状腺乳头状癌侵袭转移的有效靶点。
Objective: To investigate the expression of chemokine CXCR4 in papillary thyroid carcinoma and its relationship with clinicopathological parameters. Methods: The expression of CXCR4 mRNA and protein in 72 specimens of fresh thyroid papillary carcinoma and 52 specimens of thyroid papillary carcinoma were detected by semi-quantitative RT-PCR and immunohistochemistry. Results: The positive rates of CXCR4 mRNA and protein in papillary thyroid carcinoma were 77.8% (56/72) and 76.6% (95/124), respectively, but not in thyroid benign lesions and normal thyroid tissues (P <0.01). There was a positive correlation between CXCR4 mRNA and protein expression in papillary thyroid carcinoma (r = 0.714, P <0.01). Univariate analysis showed that CXCR4 protein expression was not related to age, sex and tumor size (P> 0.05), but was closely related to tumor invasion, lymph node metastasis, distant metastasis and clinical stage (P <0.05), and CXCR4 protein positive The prognosis of the group was significantly lower than that of the CXCR4 positive group (P <0.05). CONCLUSION: CXCR4-positive papillary thyroid carcinoma has a high invasion and metastasis potential, which is one of the indicators of poor prognosis. CXCR4 may be an effective target for inhibiting the invasion and metastasis of papillary thyroid carcinoma.