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目的检测外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中CD161+T细胞的频数变化,探讨其在类风湿关节炎(RA)发生、发展中的作用。方法收集唐山市工人医院风湿病科2009年6月至2010年6月21例类风湿关节炎患者,其中男性7例,女性14例,平均年龄55岁。采用PBMCs体外活化技术,应用流式细胞术检测RA患者外周血PBMCs中CD4+CD161+T细胞的频数情况。结果与年龄、性别匹配的健康人群相,RA患者PBMCs中CD161+CD4+T和CD161+Th1细胞的百分比显著降低[(27.53±7.34)%vs(22.75±5.11)%,(P<0.05);(11.35±4.36)%vs(8.21±3.93)%,(P<0.05)],导致RA患者CD161+与CD161-Th1细胞的比率下降,二者差异有显著性[(0.76±0.29)%vs(0.60±0.30)%,(P<0.05)]。结论 RA患者滑膜炎性反应导致外周血CD161+CD4+T细胞及其Th1亚群与相应阴性T细胞群体的平衡紊乱,CD161+CD4+T细胞及其Th1亚群在RA的病理过程密切相关。
Objective To detect the frequency of CD161 + T cells in peripheral blood mononuclear cells (PBMCs) and to explore its role in the development of rheumatoid arthritis (RA). Methods Twenty-one patients with rheumatoid arthritis from June 2009 to June 2010 were collected from Department of Rheumatology, Tangshan Workers Hospital, including 7 males and 14 females, with an average age of 55 years. Using PBMCs activation in vitro, the frequency of CD4 + CD161 + T cells in peripheral blood of RA patients was detected by flow cytometry. Results The percentage of CD161 + CD4 + T and CD161 + Th1 cells in PBMCs of patients with RA was significantly lower than that in healthy subjects matched with age and sex ([(27.53 ± 7.34)% vs (22.75 ± 5.11)%, P <0.05]. (11.35 ± 4.36)% vs (8.21 ± 3.93)%, respectively (P <0.05). The ratio of CD161 + to CD161-Th1 cells in RA patients was significantly lower than that in RA patients [(0.76 ± 0.29)% vs ± 0.30)%, (P <0.05)]. Conclusions The synovial inflammatory response in patients with RA causes the imbalance of CD161 + CD4 + T cells and their Th1 subpopulations in peripheral blood and the corresponding negative T cell population. The pathological process of CD161 + CD4 + T cells and their Th1 subsets is closely related to RA .