论文部分内容阅读
目的探讨金属蛋白酶-9(MMP-9)、埃兹蛋白(Ezrin)在宫颈上皮内瘤样病变(CIN)和宫颈鳞癌(SCC)中的表达及其相关性。方法选择手术切除的SCC组织标本47份、宫颈上皮内瘤样病变(CIN)标本35份,正常宫颈组织(NCE)21份,采用免疫组化SP法检测各组MMP-9、Ezrin的表达和微血管密度(MVD)(CD44标记)。结果SCC、CIN组中MMP-9、Ezrin阳性率和MVD值均明显高于NCE组(P<0.05),SCC组明显高于CIN组(P<0.05);MMP-9、Ezrin阳性表达均与SCC组的临床分期、组织分化程度、有无淋巴结转移相关,并且在SCC组中两者表达呈正相关;SCC组中MMP-9、Ezrin阳性者的MVD值明显高于其阴性者(P<0.01),在有无淋巴结转移者中MMP-9、Ezrin阳性者的MVD值差异有统计学意义(P<0.05),MMP-9阳性者的MVD值与分化程度有关(P<0.05)。结论 MMP-9、Ezrin在SCC发生、发展中可能起重要作用,MMP-9、Ezrin及MVD值与SCC的浸润转移密切相关,可作为预后的判断指标。
Objective To investigate the expression and correlation of MMP-9, Ezrin in cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (SCC). Methods 47 specimens of SCC, 35 specimens of cervical intraepithelial neoplasia (CIN) and 21 specimens of normal cervical tissue (NCE) were collected. The expressions of MMP-9 and Ezrin in each group were detected by immunohistochemistry Microvessel density (MVD) (CD44 marker). Results The positive rates of MMP-9, Ezrin and MVD in SCC and CIN groups were significantly higher than those in NCE group (P <0.05), while those in SCC group were significantly higher than those in CIN group (P <0.05) There was a positive correlation between the expression of MMP-9 and Ezrin in SCC group, clinical stage, histological differentiation and lymph node metastasis in SCC group, and positive expression in SCC group (P <0.01) ). There was significant difference in MVD between MMP-9 and Ezrin positive patients with and without lymph node metastasis (P <0.05). The MVD of MMP-9 positive patients was related with the degree of differentiation (P <0.05). Conclusions MMP-9 and Ezrin may play an important role in the occurrence and development of SCC. The expressions of MMP-9, Ezrin and MVD are closely related to the infiltration and metastasis of SCC, which may be used as a prognostic indicator.