目的：探讨在神经导航定位下模拟对侧经纵裂胼胝体锁孔入路的可行性。方法成人尸头湿标本5具，分别行神经导航下对侧经纵裂胼胝体锁孔入路和常规对侧经纵裂胼胝体入路。观察两种入路中的重要解剖结构，比较两者的解剖测量资料。结果对侧经纵裂胼胝体锁孔入路下侧脑室外侧壁显露范围为（1.83±0.35） cm2，与常规入路的（1.86±0.51） cm2比较无统计学差异（P＞0．05）。结论利用锁孔技术的“鱼眼效应”，在相同的显露范围下，对侧经纵裂胼胝体锁孔入路较常规入路的损伤明显减少，适用于侧脑室体部和外侧壁病变的手术。“,”Objective To investigate the indications and feasibility of the contralateral interhemispheric transcallosal keyhole approach guided by neuronavigation .Methods Guided by neuronavigation ,simulated surgery via contralateral interhemispheric transcallosal keyhole approach and traditional approach was performed in 6 adult cadaver heads fixed by formalin .Anatomic structure via two approaches was observed and anatomic data measured were compared .Results The exposure scope of the lateral wall of the lateral ventricles via the contralateral interhemispheric transcallosal keyhole approach was (1.83 ± 0.35) cm2 ,which was similar to (1.86 ± 0.51) cm2 of via traditional approach ( P>0 .05 ) . Conclusion Compared to the traditional approach , the contralateral interhemispheric transcallosal keyhole approach is feasible for accessing and treating the lesions located on the lateral wall and body of the lateral ventricles with less injury .