论文部分内容阅读
目的:建立木香顺气丸(陈皮,厚朴,苍术等)甲醇提取物指纹图谱的HPLC指纹图谱分析条件,为木香顺气丸的内在质量评价积累数据,为优化及评价中药复方指纹图谱提供一种方法。方法:采用HPLC-UV检测,ZORBAX Eclipse XDB-C18(4.6 mm×150 mm,5μm)色谱柱,乙腈-水为流动相,在35℃柱温下采用梯度洗脱,流速1 mL·min-1;检测波长280 nm,225 nm(木香);进样量5μL;实验比较了7个厂家和自制木香顺气丸的高效液相指纹图谱,并考察了单味药材的高效液相指纹图谱。结果:该方法在104～520 mg·L-1线性关系良好,Y=70 580X+1.1875,r=0.999 7(和厚朴酚);木香顺气丸甲醇提取物各组分分离较佳,精密度、稳定性、重复性好,相对保留时间RSD小于0.5%。结论:表明HPLC指纹图谱方法重现性好,稳定性可靠,可用于木香顺气丸的质量控制方法,不同厂家木香顺气丸质量有差异,而药材是影响木香顺气丸质量差异的因素之一。
OBJECTIVE: To establish HPLC fingerprint of methanol extracts of Muxiang Shunqi Pill (Citrus rehmanniae, Magnolia officinalis, Cangzhu, etc.) and to accumulate data for evaluating the inherent quality of Muxiang Shunqi Pills. In order to optimize and evaluate the fingerprint of TCM compound fingerprints Provide a method. Methods: The HPLC-UV method was used. The mobile phase consisted of a mobile phase of ZORBAX Eclipse XDB-C18 (4.6 mm × 150 mm, 5 μm) with acetonitrile-water and gradient elution with a flow rate of 1 mL · min -1 ; Detection wavelength 280 nm, 225 nm (woody); injection volume 5μL; experimental comparison of high-performance liquid fingerprint of seven manufacturers and homemade Muxiang Shunqi pills, and investigated the single-drug HPLC fingerprint . Results: The method showed good linearity at 104-520 mg · L-1, Y = 70 580X + 1.1875, r = 0.999 7 (honokiol) Precision, stability, repeatability, relative retention time RSD less than 0.5%. Conclusion: The HPLC fingerprinting method has good reproducibility and reliability. It can be used in the quality control method of Muxiang Shunqi Pill. The quality of Muxiang Shunqi Pill differs from manufacturer to manufacturer. However, One of the factors.