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目的:探讨血管紧张素转换酶(ACE)基因插入/缺失(I/D)和醛固酮合成酶(CYP11B2)基因-344T/C多态性及环境因素与高血压病(EH)中风先兆证的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法,检测116例EH中风先兆证患者和与之1∶1相匹配(按性别、年龄±3岁、居住地)的正常对照组CYP11B2-344T/C基因型,同时用PCR法检测ACE(I/D)基因型。结果:ACE-DD基因型频率在EH中风先兆证组显著高于对照组(P<0.05),CYP11B2-TT基因型频率分布无差异(P>0.05)。多因素条件Logistic回归筛选出荤食、急躁易怒及ACE-DD基因型为主要危险因素,而ACE-DD型OR为2.834(95%CI,1.232-6.518)。结论:ACE基因I/D多态性与EH中风先兆证显著关联,此关系在调整了中医传统危险因素后依然存在,并提示在发病的过程中是环境与遗传共同作用的结果。
Objective: To investigate the relationship between ACE gene insertion / deletion (I / D) and aldosterone synthase (CYP11B2) gene -344T / C polymorphism and environmental factors and stroke-threatening stroke in patients with essential hypertension (EH) . Methods: One hundred and sixteen patients with stroke-threatening stroke in EH were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and matched with 1: 1 (sex, age ± 3 years, place of residence) The CYP11B2-344T / C genotypes were detected in the normal control group, while ACE (I / D) genotypes were detected by PCR. Results: The frequency of ACE-DD genotype was significantly higher in the EH-induced stroke group than in the control group (P <0.05). There was no difference in the frequency distribution of CYP11B2-TT genotype (P> 0.05). Multivariate conditional Logistic regression screened meat food, irritability and ACE-DD genotype as the main risk factors, while ACE-DD type OR was 2.834 (95% CI, 1.232-6.518). CONCLUSION: ACE gene I / D polymorphism is significantly associated with EH stroke precursory syndrome. This relationship still exists after adjusting traditional Chinese medicine risk factors and suggests that the relationship between environment and heredity results in the pathogenesis.