Objective:To investigate the expression and the regulation effect of cell growth of microRNA-577 in hepatocellular carcinoma(HCC).Methods:qRT-PCR was applied to detect the relative expression of miR-577 in 70 paired HCC and matched tumor adjacent tissues collecting from resection between March 2011 and March 2014.Pearson chi-square test was used to analyze the relationship between the miR-577 expression and clinical features.The miR-577 mimics were transfected into HepG2 cells:cell cycles were detected by flow cytometry,cell proliferation was measured by MTT assay and BrdU incorporation assay,and cell apoptosis was determined by flow cytometry and Caspase 3/7 activity analysis.The expressions ofβ-catenin were measured by immunohistochemistry.Spearman correlation analysis was used to analyze the relationship between miR-577 and p-catenin.qRT-PCR and westernblot were used to detect the expression of p-catenin in transfected HepG2 cells.Results:The relative expressions of miR-577 was significantly lower in HCC tissues compared to the matched normal tumor-adjacent tissues(P<0.05).Low expression of miR-577 was significantly associated with large tumor size(≥5 cm,P<0.05) and advanced tumor node metastasis stage(Ⅲ+Ⅳ,P<0.05).Transfection of miR-577 mimics could inhibit repress cell proliferation,enhance cell apoptosis and block the cell cycles in G_1/G_1 phase(P<0.05).miR-577 in HCC group had a significant negative correlation relationship with the expression of downstream target of β-catenin(P<0.05).Both the mRNA and protein expression in HepG2 cells were down-regulated after transfection(P<0.05).Conclusions:Low expression of miR-577 is related to the malignant clinicopathological features in HCC tissues,and miR-577 may suppress HCC growth through down-regulating p-catenin. Objective: To investigate the expression and the regulation effect of cell growth of microRNA-577 in hepatocellular carcinoma (HCC). Methods: qRT-PCR was applied to detect the relative expression of miR-577 in 70 paired HCC and matched tumor adjacent tissues collecting from resection between March 2011 and March 2014. Pearson chi-square test was used to analyze the relationship between the miR-577 expression and clinical features. The miR-577 mimics were transfected into HepG2 cells: cell cycles were detected by flow cytometry, cell proliferation was measured by MTT assay and BrdU incorporation assay, and cell apoptosis was determined by flow cytometry and Caspase 3/7 activity analysis. expressions of β-catenin were measured by immunohistochemistry. pearman correlation analysis was used to analyze the relationship between miR-577 and p-catenin.qRT-PCR and western blot were used to detect the expression of p-catenin in transfected HepG2 cells. Results: The relative expressions of miR-577 was sig nificantly lower in HCC tissues compared to the matched normal tumor-adjacent tissues (P <0.05) .Low expression of miR-577 was significantly associated with large tumor size (≥5 cm, P <0.05) and advanced tumor node metastasis stage + Ⅳ, P <0.05) .Transfection of miR-577 mimics could inhibit repress cell proliferation, enhance cell apoptosis and block the cell cycles in G_1 / G_1phase (P <0.05) .miR-577 in HCC group had a significant negative correlation Relationship between the expression of downstream target of β-catenin (P <0.05) .Both the mRNA and protein expression in HepG2 cells were down-regulated after transfection (P <0.05) .Conclusions: Low expression of miR-577 is related to the malignant clinicopathological features in HCC tissues, and miR-577 may suppress HCC growth through down-regulating p-catenin.