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采用高分辨质谱及代谢物鉴定软件,系统分析了猪尿液和血浆中沙丁胺醇代谢产物。样品用乙腈沉淀蛋白,经离心过滤后,采用Agilent Plus C18色谱柱,以乙腈和醋酸溶液(含0.2%醋酸)作为流动相进行梯度洗脱,采用高分辨质谱(UHPLC-QTOF MS)±离子模式进行检测,利用软件Agilent MassHunter MetaboliteID与人工相结合分析检测数据。结果表明,经灌胃给药后猪尿液中鉴定出沙丁胺醇原形药和葡糖醛酸化、苯环羟基化、N-氧化、苯环甲氧基化、羟基化脱水共5个代谢产物,其中2个代谢物未见文献报道;而血浆中仅检测原型药物和2个代谢物,包括沙丁胺醇的葡糖醛酸化和N-氧化产物。
High-resolution mass spectrometry and metabolite identification software were used to systematically analyze salbutamol metabolites in urine and plasma. The sample was precipitated with acetonitrile and centrifuged. The residue was subjected to gradient elution on an Agilent Plus C18 column with acetonitrile and acetic acid (containing 0.2% acetic acid) as the mobile phase. High resolution mass spectrometry (UHPLC-QTOF MS) ion mode The tests were performed using the software Agilent MassHunter MetaboliteID in conjunction with the manual analysis of assay data. The results showed that five metabolites of albuterol and glucuronidation, hydroxylation of benzene ring, N-oxidation, methoxylation of benzene ring and dehydration of hydroxylation were identified in swine urine, Two metabolites were not reported in the literature; only the prototype drug and two metabolites were detected in plasma, including glucuronidation and N-oxidation of albuterol.