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应用细胞免疫荧光技术和流式细胞术对32例子宫内膜癌，26例子宫内膜癌前病变和10例正常子宫内膜组织进行了ras癌基因产物P21蛋白和DNA倍体的检测。结果发现：在正常子宫内膜组织中无P21蛋白的表达，子宫内膜癌P21的表达高于癌前病变，P＜0．05。P21蛋白的表达荧光指数随组织学分级的升高而增加，并与DNA倍体有关。但与临床分期，肌层浸润深度和淋巴结转移无关。说明P21蛋白与子宫内膜癌的发生有关。临床检测P21蛋白和DNA倍体有利于判断子宫内膜癌的恶性程度和预后。
The ras oncogene product P21 protein and DNA ploidy were detected by cellular immunofluorescence and flow cytometry in 32 cases of endometrial carcinoma, 26 cases of endometrial precancerous lesions and 10 cases of normal endometrium. The results showed that there was no expression of P21 protein in normal endometrium. The expression of P21 in endometrial carcinoma was higher than that in precancerous lesions, P<0.05. The fluorescence index of P21 protein expression increases with histological grade and is related to DNA ploidy. However, with clinical staging, there was no correlation between depth of muscular invasion and lymph node metastasis. It shows that P21 protein is involved in the occurrence of endometrial cancer. Clinical detection of P21 protein and DNA ploidy is helpful to determine the degree of malignancy and prognosis of endometrial cancer.