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本研究以NaF 0、30、60,90mg/kg灌胃,观察大鼠48小时急性氟中毒尿氟排泄规律、肾损害情况及其与LPO的关系。结果发现:中毒大鼠尿氟浓度在中毒3小时显著升高,中毒6小时达高峰;肾损害指标尿AKP活性显著增强,峰值分别在12、24小时;尿蛋白含量显著升高,峰值在36小时;肾匀浆MDA含量显著升高,肾匀浆GSH含量显著降低,肾G—6—Pase活性显著下降,以上三指标均存在显著的剂量效应关系,肾SDHase活性显著降低。提示氟中毒可损害肾脏;其损害的机理之一是LPO;损害亚细胞部位可能在线粒体和微粒体。
In this study, NaF 0, 30, 60 and 90 mg / kg were intragastrically fed to observe the regularity of urinary fluoride excretion, renal damage and their relationship with LPO in rats with acute fluorosis at 48 hours. The results showed that the concentration of urinary fluoride in the poisoned rats increased significantly at 3 hours and reached the peak at 6 hours. Urinary AKP activity increased significantly at 12 and 24 hours after renal injury, while urine protein content increased significantly at 36 Hour, the content of MDA in kidney homogenate increased significantly, the content of GSH in renal homogenate decreased significantly, and the activity of G-6-Pase in kidney decreased significantly. There was a significant dose-effect relationship among the above three indexes, and the activity of SDHase in kidney decreased significantly. Prompted fluorosis can damage the kidneys; one of the mechanisms of damage is LPO; damage to subcellular parts may be mitochondria and microsomes.