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本文报道了一种改进的分化染色法,它通过细胞核的颜色很容易鉴别出在培养中已经发生分裂的细胞。只有在这些细胞里才能观察到微核,并证实当不计数未分裂的淋巴细胞时,微核分析对于淋巴细胞培养中的干扰敏感性很小。从健康成人静脉穿刺取0.3ml血作全血培养。BrdU最终浓度为4×10~(-4)μ,在37℃黑暗中培养76小时。终止培养前2小时加入秋水仙胺。
This article reports an improved differentiation staining method that readily distinguishes cells that have already undergone division in culture by the color of the nucleus. Only micronuclei can be observed in these cells and demonstrate that micronucleus analysis is less sensitive to interference in lymphocyte culture when non-dividing lymphocytes are not counted. Take 0.3ml blood from healthy adult venipuncture for whole blood culture. The final concentration of BrdU was 4 x 10 ~ (-4) μ and cultured in the dark at 37 ° C for 76 hours. Colchicine was added 2 hours prior to termination of culture.