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目的:观察喘消雾化液(CXW)对博莱霉素(BLM)诱导大鼠肺纤维化模型的影响,为肺纤维化的防治提供实验依据。方法:90只雄性Wistar大鼠随机分为生理盐水(NS)组、模型组(BLM组)、CXW组。BLM、CXW组经腹腔注入15mg/kgBLM×10d制作肺纤维化动物模型,分别于第1、14、28天每组各处死10只,留取血液,收集支气管肺泡灌洗液(BALF)进行细胞学计数及其分类;评价各组各时间点肺组织病理形态学变化,定量肺泡间隔宽度;检测肺组织匀浆中羟脯氨酸(HYP)含量;检测血清和肺组织匀浆中超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果:(1)与NS组比较,BLM组在第1、14、28天时的肺泡壁厚度、胸膜厚度和肺系数差异均有统计学意义(P均<0.05),CXW组肺泡壁厚度、胸膜厚度明显增加,与BLM组比较差异有统计学意义(P均<0.05)。(2)与同期NS组比较,BLM组第14、28天的HYP含量,第1、14天的细胞总数及炎性细胞比例和肺组织匀浆中MDA含量均明显升高(P均<0.05),第1天肺组织匀浆中SOD活力明显降低(P<0.05)。同期CXW组改变不明显,与BLM组比较,差异均有统计学意义(P均<0.05)。结论:CXW可能通过提高大鼠肺纤维化模型中SOD活力,降低MDA含量,并在一定程度上阻止或延缓了胶原的沉积,起到了保护肺组织的作用。
Objective: To observe the effect of CXW on BLM-induced pulmonary fibrosis in rats and provide experimental evidence for the prevention and treatment of pulmonary fibrosis. Methods: Ninety male Wistar rats were randomly divided into saline group (NS), model group (BLM group) and CXW group. BLM and CXW groups were injected intraperitoneally with 15 mg / kg BLM for 10 days to make animal models of pulmonary fibrosis. Ten rabbits in each group were killed on the 1st, 14th and 28th day respectively. Blood was collected and bronchoalveolar lavage fluid (BALF) The counts of each group and its classification were evaluated. The histopathological changes of lung tissues were evaluated at each time point to determine the width of the alveolar septum. The levels of hydroxyproline (HYP) in the lung homogenates were measured. The levels of superoxide dismutase Enzyme (SOD) activity and malondialdehyde (MDA) content. Results: (1) Compared with NS group, the alveolar wall thickness, pleural thickness and pulmonary coefficient in BLM group were significantly different on the 1st, 14th and 28th days (all P <0.05) The thickness increased significantly, compared with the BLM group, the difference was statistically significant (P all <0.05). (2) Compared with the NS group, the HYP content, the total number of cells on day 1 and 14, the proportion of inflammatory cells and the content of MDA in lung homogenate of BLM group were significantly increased on the 14th and 28th day (all P <0.05 ), The activity of SOD in lung tissue homogenate was significantly decreased on the first day (P <0.05). There was no significant change in the CXW group during the same period, compared with the BLM group, the differences were statistically significant (all P <0.05). CONCLUSION: CXW may protect the lung tissue by increasing the activity of SOD and decreasing the content of MDA in rat pulmonary fibrosis model, and to a certain extent, prevent or delay the deposition of collagen.