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背景:研究手机微波是否对人体健康有潜在危害已成为热点问题。目的:观察不同频率和不同发射功率的微波照射对小鼠脑丙二醛含量、乳酸脱氢酶(lactatedehydrogenase,LDH)和一氧化氮合酶(nitricoxidesynthase,NOS)活性的影响。设计:随机对照的实验研究。地点和对象:实验地点:首都医科大学动物科学部。按随机数字表法将50只无特定病原体(SPF)C57BL/6N小鼠分为正常对照组和45MHz组(新式手机组):照射功率20mW/cm2,照射时间12h/d;450MHz组(老式手机组):照射功率100mW/cm2,照射时间6h/d;870MHz组(无绳电话组):照射功率100mW/cm2,照射时间6h/d;2450MHz(强微波组):照射功率730mW/cm2,照射时间2s/次,3次/d。每组10只。干预:采用不同辐照强度的微波照射3个月后颈椎脱臼处死,用硫代巴比妥酸(TBA)比色法、二硝基苯肼显色法(DNPH比色法)和吩嗪二甲脂比色法(PMS显色法)分别检测小鼠脑组织中脂质过氧化产物-丙二醛含量、LDH和NOS活性。主要观察指标:①强微波辐照时小鼠脑中丙二醛含量、LDH和NOS的活性。②弱微波辐照时小鼠脑中丙二醛含量和LDH的活性。结果:2450MHz强微波辐射后,丙二醛含量犤(29.571±3.888)μmol/g犦升高,与对照组犤(21.660±2.729)μmol/g犦比较,差异有统计学意义(Z=1.789,P<0.01),LDH和NOS活性犤(17.56
Background: It has become a hot issue to study whether mobile-phone microwaves have a potential harm to human health. OBJECTIVE: To observe the effect of microwave irradiation with different frequencies and different transmit power on malondialdehyde content, lactate dehydrogenase (LDH) and nitric oxide synthase (NOS) activity in mice brain. Design: Randomized controlled experimental study. Venue and Subject: Experimental Location: Department of Animal Science, Capital Medical University. 50 SPF-free C57BL / 6N mice were divided into normal control group and 45MHz group (new cell phone group) according to random number table method: irradiation power of 20mW / cm2 and irradiation time of 12h / d; 450MHz group Group): irradiation power 100mW / cm2, irradiation time 6h / d; 870MHz group (cordless telephone group): irradiation power 100mW / cm2, irradiation time 6h / d; irradiation power of 2450MHz (strong microwave group): irradiation power 730mW / cm2, irradiation time 2s / time, 3 times / d. Each group of 10. Intervention: Cervical dislocations were sacrificed 3 months after microwave irradiation with different irradiation intensities. Thiobarbituric acid (TBA) colorimetry, dinitrophenylhydrazine colorimetry (DNPH colorimetric method) and phenazine bis Methyl lipid colorimetry (PMS) was used to detect the lipid peroxidation products (MDA), LDH and NOS activity in mouse brain. MAIN OUTCOME MEASURES: ① Malondialdehyde content, LDH and NOS activity in mouse brain during intense microwave irradiation. ② The content of malondialdehyde and the activity of LDH in mouse brain when exposed to weak microwave radiation. Results: After 2450MHz intense microwave radiation, the content of malondialdehyde (.5 (29.571 ± 3.888) μmol / g 犦 increased, compared with the control group 犤 (21.660 ± 2.729) μmol / g,, the difference was statistically significant P <0.01), LDH and NOS activity (17.56