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目的了解广州某医院秋冬季儿童腹泻中人类杯状病毒(HuCV)感染的分子流行病学特点。方法连续2个秋冬季腹泻流行季节收集临床诊断为病毒性腹泻患儿的粪便标本。采用ELISA检测轮状病毒,RT-PCR检测HuCV。部分阳性标本PCR产物进行纯化、测序,结合参考株相应核苷酸序列进行进化分析。结果诺瓦克样病毒(Norwalk-likevirus,NLVs)阳性率为12.35%(80/648),扎幌样病毒(Sapporo-likevirus,SLVs)阳性率为0.31%(2/648)。NLVs各月阳性率无显著性差异,主要感染2岁以下患儿。2003年流行株为GⅡ-3群和GⅡ-4群,2004年为GⅡ-4群。2株SLVs均为GⅠ-1群。结论NLVs是广州地区秋冬季节儿童腹泻的重要病原体,其流行株为GⅡ-3群、GⅡ-4群病毒株,但在不同的时间段可能存在不同的流行优势株;广州地区存在SLVs所致感染,与我国报告的SLV基因型不同。
Objective To understand the molecular epidemiology of human calicivirus (HuCV) infection in children’s diarrhea in autumn and winter in a hospital in Guangzhou. Methods Two fecal specimens of children with viral diarrhea were collected during the two seasons of autumn and winter diarrhea. Rotavirus was detected by ELISA and HuCV by RT-PCR. The PCR products of some positive samples were purified and sequenced, and the corresponding nucleotide sequences of reference strains were analyzed for evolution. Results The positive rate of Norwalk-like virus (NDVs) was 12.35% (80/648), and the positive rate of Sapporo-like virus (SLVs) was 0.31% (2/648). There was no significant difference in the positive rates of NLVs every month, mainly affecting children under 2 years old. 2003 epidemic strains of G Ⅱ-3 and G Ⅱ-4 group, 2004 G Ⅱ-4 group. Two strains of SLVs were GⅠ-1 group. Conclusions NLVs is an important pathogen of childhood diarrhea in autumn and winter in Guangzhou. The epidemic strains are GⅡ-3 group and GⅡ-4 group, but different predominant strains may exist at different time points. SLVs-related infections exist in Guangzhou , Different from the reported SLV genotype in our country.