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大蒜辣素是国际公认的大蒜活性物质,具有心血管保护作用。该实验主要观察大蒜辣素对大鼠心肌梗死后纤维化的影响,并探讨其作用与TGFβ/Smads信号通路的关系。实验采用结扎大鼠冠状动脉左前降支的方法制备大鼠心肌梗死模型,术后24 h开始腹腔注射给药,给药21 d后处死动物,取心脏组织做Masson染色观察心肌组织纤维化情况,免疫组织化学方法观察心肌组织Ⅰ,Ⅲ型胶原蛋白表达情况及TGFβ1,Smad3和Smad7蛋白表达情况。结果显示模型组大鼠心肌纤维化明显,Ⅰ,Ⅲ型胶原表达量增加,大蒜辣素各剂量组均可减轻心肌纤维化程度,减少Ⅰ,Ⅲ型胶原表达,表明大蒜辣素可抑制心肌梗死后心肌纤维化的进展。同时大蒜辣素还可下调心肌TGFβ1和Smad3蛋白表达,上调Smad7蛋白表达。该研究揭示了大蒜辣素具有抗心肌梗死后纤维化作用,其机制部分与调节TGFβ/Smads信号通路有关。
Allicin is an internationally recognized garlic active substance with cardiovascular protection. This experiment mainly observed the effect of allicin on fibrosis after myocardial infarction in rats, and explored the relationship between TGFβ / Smads signaling pathway and its role. The model of myocardial infarction was established by ligation of the left anterior descending coronary artery in rats. The rats were injected intraperitoneally 24 hours after the operation and the animals were sacrificed 21 days after the administration. Masson staining was used to observe the myocardial fibrosis, Immunohistochemistry was used to observe the expression of type Ⅰ and type Ⅲ collagen and the expression of TGFβ1, Smad3 and Smad7 in myocardium. The results showed that myocardial fibrosis in model group was obvious, the expression of type I and type III collagen increased, allicin dose groups could reduce the degree of myocardial fibrosis and reduce type I and type III collagen expression, indicating that allicin can inhibit myocardial infarction After myocardial fibrosis progress. At the same time allicin can also down-regulate TGFβ1 and Smad3 protein expression, up-regulate Smad7 protein expression. This study revealed that allicin has anti-fibrotic effects after myocardial infarction, and its mechanism is partially related to the regulation of TGFβ / Smads signaling pathway.