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采用流式细胞仪,3H-TdR掺入和酶联免疫打点(enzyme-linked immunospot,ELISPOT)方法,研究妊娠免疫学指标的改变。妊娠晚期大鼠脾脏单个核细胞表面分子主要组织相容性抗原Ⅱ(MHdCⅡ)明显下调,外周血单个核细胞表达CD11c明显减少,共激活分子B7-1和B7-2未见改变;脾脏和外周血单个核细胞中Th2细胞因子IL,10、IL-4表达增多,TGFB阳性细胞数也明显增加,而Th1细胞因子IFNγ的产生未受抑制。此外,脾脏和外周血中单个核细胞的抗原特异性增殖未见改变,而腹腔淋巴结细胞的增殖明显升高。脾脏单个核细胞在妊娠晚期分泌较少的抗原特异性抗体。提示妊娠期性激素具有免疫调节作用,可能与怀孕时Th1细胞介导的自身免疫性疾病得到缓解有关。
Flow cytometry, 3H-TdR incorporation and enzyme-linked immunospot (ELISPOT) method were used to study the change of pregnancy immunological parameters. In the third trimester of pregnancy, the major histocompatibility antigen (MHDCⅡ) on the surface of mononuclear cells in the third trimester of pregnant rats was significantly down-regulated, while the expression of CD11c in peripheral blood mononuclear cells was significantly decreased. The co-activators B7-1 and B7-2 showed no changes. Th2 cytokines IL-10, IL-4 in blood mononuclear cells increased, the number of TGFB positive cells also increased significantly, while the production of Th1 cytokine IFNγ was not inhibited. In addition, the antigen-specific proliferation of mononuclear cells in spleen and peripheral blood was unchanged, while the proliferation of peritoneal lymph node cells was significantly increased. Spleen mononuclear cells secrete less antigen-specific antibodies in late pregnancy. Suggesting that sex hormones during pregnancy with immunomodulatory effects, may be associated with Th1 cell-mediated autoimmune diseases alleviated during pregnancy.