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AIM: To establish a LC-MS/MS method for determination of Pravastatin in human plasma. METHODS: After addition of rosuvastatin (internal standard) with liquid-liquid extraction, the analysis involved an Intersil ODS-3(2.1 mm×50 mm,5 μm)column. The mobile phase consisted of acetonitrile:1 mmol ammonium formate (include 0.1% formic acid)(60∶40,v/v),the flow rate was 0.2 mL/min, the column temperature was set at 40 ℃. ESI+was performed in the MRM mode using target ions at m/z 448-328(Pravastatin)and m/z 483-271(rosuvastatin). RESULTS: The peak area of Pravastatin in plasma showed good linearity within the range between 0.957-510.000 ng/mL,r2=0.999. The lower limit of quantitation (LLOQ) of pravastatin in plasma was 0.957 ng/mL. The extracted recovery was >75%, The relative recovery was 99%-115%,the intra-and inter-day RSD were <15%. CONCLUSION: This method is sensitive,simple and accurate, it can be used for determination of Pravastatin sodium tablets serum concentrations.
METHODS: After addition of rosuvastatin (internal standard) with liquid-liquid extraction, the analysis involved an Intersil ODS-3 (2.1 mm × 50 mm, The mobile phase consisted of acetonitrile: 1 mmol ammonium formate (including 0.1% formic acid) (60:40, v / v), the flow rate was 0.2 mL / min, . ESI + was performed in the MRM mode using target ions at m / z 448-328 (Pravastatin) and m / z 483-271 (rosuvastatin). RESULTS: The peak area of Pravastatin in plasma showed good linearity within the range between 0.957 -510.000 ng / mL, r2 = 0.999. The lower limit of quantitation (LLOQ) of pravastatin in plasma was 0.957 ng / mL. The extracted recovery was> 75%, The relative recovery was 99% -115%, the intra- and Inter-day RSD were <15%. CONCLUSION: This method is sensitive, simple and accurate, it can be used for determination of Pravastatin sodium tablets serum concentrations.