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Gas chromatography-mass spectrometry(GC-MS) and the chemometric resolution method(alternative moving window factor analysis,AMWFA) were used for comparative analysis of volatile constituents in herbal pair(HP) flos lonicerae-caulis lonicerae(FL-CL) and its single herbs.The temperature-programmed retention index(PTRI) was also employed for the identification of compounds.In total,44,39,and 50 volatile chemical components in volatile oil of FL,CL and HP FL-CL were separately determined qualitatively and quantitatively,accounting for 87.22%,94.54% and 90.08% total contents of volatile oil of FL,CL and HP FL-CL,respectively.The results show that there are 32 common volatile constituents between HP FL-CL and single herb FL,33 common volatile constituents between HP FL-CL and single herb CL,and 10 new constituents in the volatile oil of HP FL-CL.
Gas chromatography-mass spectrometry (GC-MS) and the chemometric resolution method (alternative moving window factor analysis, AMWFA) were used for comparative analysis of volatile constituents in herbal pair (HP) flos lonicerae-caulis lonicerae (FL-CL) single herbs. The temperature-programmed retention index (PTRI) was also employed for the identification of compounds. In total, 44, 39, and 50 volatile chemical components in volatile oil of FL, CL and HP FL- quantitatively, accounting for 87.22%, 94.54% and 90.08% total contents of volatile oil of FL, CL and HP FL-CL, respectively. These results show that there are 32 common volatile constituents between HP FL-CL and single herb FL, 33 common volatile constituents between HP FL-CL and single herb CL, and 10 new constituents in the volatile oil of HP FL-CL.