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用82-1培养基对14份糯玉米和10份优质蛋白玉米的花培反应率进行了离体培养研究。结果表明,14份糯玉米材料中有13份具有花培反应,占参试糯玉米材料的92.86%,其中反应率最高的是作登糯玉米(出愈率3.16%);10份优质蛋白玉米材料中有5份具有花培反应,占参试优质蛋白玉米材料的50.00%,其中反应率最高的是CML170(出愈率0.65%)。3份糯玉米材料即田东作登糯、都安良昌糯、天峨糯能产生正常绿苗,占参试糯玉米材料的21.43%,正常绿苗率分别为4.08%、9.09%、25.00%;1份优质蛋白玉米材料(CML170)产生正常绿苗,正常绿苗率占参试优质蛋白玉米材料的10.00%。试验结果还表明,糯玉米材料的出愈率以及绿苗率均比优质蛋白玉米材料高;糯玉米与优质蛋白玉米的花培反应同普通玉米一样存在基因型依赖性。
In vitro culture of 14 waxy maize and 10 high quality protein maize cultivars was conducted in 82-1 medium. The results showed that 13 of the 14 waxy maize materials had the flower culture reaction, accounting for 92.86% of the waxy maize materials tested. The highest response rate was Daedong waxy corn (3.16% of the callus); 10 high quality protein maize Among the materials, 5 cultivars had the flower culture reaction, accounting for 50.00% of the tested high quality protein corn material, of which the highest response rate was CML170 (the outbreak rate was 0.65%). The three waxy corn materials, Tiandongzuo glutinous rice, Dianliangchang glutinous rice and Tiantanuo glutinous rice produced normal green seedlings, accounting for 21.43% of the waxy corn cultivars tested. The normal green plantlet rates were 4.08%, 9.09%, 25.00% ; 1 high quality protein corn material (CML170) produced normal green seedlings, and the normal green seedling rate accounted for 10.00% of the tested high quality protein corn material. The test results also show that the waxy corn and the green seedling rate are higher than the high quality protein corn material; Glutinous corn and high quality protein flower culture reaction with the same as the common corn genotype-dependent.