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本文建立了快速、简便测定人血清中氨氯地平的ＨＰＬＣ法，分析柱为 ＯＤＳ－５Ｓ（ １５０ × ４．０ｍｍ）不锈钢柱，流动相为甲醇：０．０３Ｍ磷酸二氢钾缓冲液（９∶１）的混合液。流速为１．０ｍｌ／ｍｉｎ，以硝苯地平为内标物，检测波长为３６６ｎｍ，蛋白沉淀剂选用１５％高氨酸。氨氯地平测定的线性范围为４～１２μｇ／ｍｌ，ｒ＝０．９９９２，最低检测限度为 ４ｎｇ。日内平均相对回收率为 １０．２４ ± ５．４０％，日间平均相对回收率为 ９９．８７ ± ６．２３％。本方法专一性好，放置２４ｈ对含量测定无影响。
In this paper, a rapid and simple HPLC method for the determination of amlodipine in human serum was established. The analytical column was ODS-5S (150 × 4.0 mm) stainless steel column. The mobile phase consisted of methanol: 0.03 M potassium dihydrogen phosphate buffer (9: 1). Flow rate of 1.0ml / min to nifedipine as the internal standard, the detection wavelength of 366nm, 15% selection of protein precipitator of high-acid. Amlodipine determination of the linear range of 4 ~ 12μg / ml, r = 0.9992, the minimum detection limit of 4ng. The average relative recovery during the day was 10.24 ± 5.40%, and the average relative recovery during the day was 99.87 ± 6.23%. Specificity of this method is good, placed 24h on the determination of no effect.