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目的:研究柴胡复方水提物对大鼠急性胃溃疡预防作用的影响。方法:大鼠灌胃给予消炎痛(70 mg/kg)复制急性胃溃疡模型,测定胃溃疡指数和溃疡抑制率,常规苏木素-伊红染色观察胃黏膜病理形态学的改变,一氧化氮硝酸还原酶法测定血清一氧化氮(NO)含量的变化,黄嘌呤氧化酶法测定血清超氧化物歧化酶(SOD)的活力,观察柴胡复方水提物对大鼠急性胃溃疡的预防作用。结果:阳性药物Stillen组(100 mg/kg)、柴胡复方水提物低剂量(100 mg/kg)和高剂量组(200 mg/kg),溃疡指数明显低于溃疡模型组(P<0.01),溃疡抑制率分别为48%、79%和80.9%,血清SOD较模型组显著降低(P<0.01);与模型组比较,柴胡复方水提物高低剂量组血清NO降低(P<0.05);胃组织病理形态学观察,模型组胃黏膜层大量缺失,严重者已脱落坏死,黏膜下层充血、水肿,且有少量纤维素、炎性细胞浸润,阳性药物组和柴胡复方高低剂量组胃组织病变有明显改善。结论:柴胡复方水提物对大鼠急性胃溃疡模型有明显的预防保护作用。
Objective: To study the preventive effect of Bupleurum chinense compound aqueous extract on acute gastric ulcer in rats. Methods: The rat model of acute gastric ulcer was induced by intraperitoneal injection of indomethacin (70 mg / kg). The gastric ulcer index and ulcer inhibition rate were measured. The pathological changes of gastric mucosa were observed by routine hematoxylin - eosin staining. Nitric oxide The change of serum nitric oxide (NO) content was measured by enzymatic method. The activity of serum superoxide dismutase (SOD) was measured by xanthine oxidase method. The preventive effect of Bupleurum chinense compound aqueous extract on acute gastric ulcer was observed. Results: The ulcer index was significantly lower in Stillen group (100 mg / kg), Bupleurum chinense compound aqueous extract (100 mg / kg) and high dose group (200 mg / kg) ), And the ulcer inhibition rates were 48%, 79% and 80.9%, respectively. Serum SOD was significantly lower than the model group (P <0.01). Compared with the model group, serum NO was lower in the high- and low- ); Gastric histopathological observation, a large number of gastric mucosa in model group was missing, severe cases have been off necrosis, submucosal congestion, edema, and a small amount of cellulose, inflammatory cell infiltration, positive drug group and Bupleurum Compound high and low dose group Stomach tissue lesions have significantly improved. Conclusion: Bupleurum chinense aqueous extract has obvious preventive and protective effects on acute gastric ulcer model in rats.