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造血微环境在引导或支持造血方面起有重要作用。但大部份仍是间接观察造血基质对造血的影响。许多报告指出,骨髓中酸性粘多糖(AMPS)增高,不利于红系而有利于粒系造血。作者注意到结缔组织成分可能对造血细胞的增殖与分化起有调节作用。因而对酸性粘多糖人工合成的葡聚糖作了实验观察。用(C57BL/Rij×CBA/Rij)F_1雄性小鼠为供体,制成含10~5/毫升骨髓细胞悬液的血浆凝块培养基(内加0.25单位/毫升EPⅢ),放0.1毫升入微皿中培养,观察红灶。粒灶则加0.1单位/毫升小鼠成纤维细胞条件培养基,用35毫米皿培养。此外,各皿分
The hematopoietic microenvironment plays an important role in guiding or supporting hematopoiesis. However, most of them still indirectly observe the influence of hematopoietic matrix on hematopoiesis. Many reports indicate that the increase in the amount of acidic mucopolysaccharides (AMPS) in the bone marrow is detrimental to erythroid and favors myeloid hematopoiesis. The authors note that connective tissue components may play a regulatory role in the proliferation and differentiation of hematopoietic cells. Therefore, artificial mucopolysaccharide artificial glucan made experimental observation. A blood plasma clot medium (containing 0.25 units / ml EPIII) containing 10-5 / ml bone marrow cell suspension was prepared using a donor of (C57BL / RijxCBA / Rij) F_1 male mice and 0.1 ml Cultivation dish, observe the red stove. Then add 0.1 units / ml of mouse fibroblast conditioned medium to the granulosa cells and culture in 35 mm dishes. In addition, each dish