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目的:利用斑马鱼模型评价和比较山豆根不同提取方法提取的有效部位的体内毒性。创新点:首次在斑马鱼模型中证明山豆根提取方法不同,有效部位的毒性有明显差异。研究结果有助于指导山豆根的新药开发与临床应用。方法:用五种不同的溶剂(去碱水、乙醇、正丁基乙醇、二氯甲烷和乙醚)提取山豆根,然后通过高效液相色谱法(HPLC)检测有效部位,将AB品系斑马鱼分为对照组(养鱼水处理)和实验组(山豆根提取物)。实验组根据采用的提取溶剂不同,分为以下六组:去碱水提取组、乙醇沉提取组、正丁基乙醇提取组、二氯甲烷提取组和乙醚提取组以及山豆根总组分组(对照),观察各种山豆根提取物对斑马鱼的急性毒性与毒性靶器官。结论:山豆根乙醚提取组诱发斑马鱼心血管毒性(图1);山豆根去碱水提取组、乙醇沉提取组以及山豆根总组分组诱发斑马鱼肝脏毒性(图3和图4);而山豆根正丁基乙醇提取组和二氯甲烷提取组诱发斑马鱼心血管毒性(图1和图2)和肝脏毒性(图3和图4)。
OBJECTIVE: To evaluate and compare the in vivo toxicity of effective fractions extracted from different extraction methods of Fructus Junnese by zebrafish model. Innovation: For the first time in the zebrafish model to prove the different extraction methods of the roots, the effective part of the significant differences in toxicity. The results of the study will help guide the development and clinical application of the new drug. Methods: The roots of Radix solanacearum were extracted with five different solvents (dealkalized water, ethanol, n-butanol, methylene chloride and diethyl ether), and the effective fractions were detected by high performance liquid chromatography (HPLC) Divided into control group (fish water treatment) and experimental group (root bean extract). The experimental group was divided into the following six groups according to different extraction solvents: de-alkali water extraction group, ethanol precipitation group, n-butanol extraction group, methylene chloride extraction group and ether extraction group, Control) to observe the acute toxicity and toxic target organs of all kinds of Rhizoma. Conclusions: Cardiotoxicity of zebrafish is induced by the extract of Euphorbia officinalis (Fig. 1), and that of the extract of Rhizoma et Radix notoginseng, ethanol extract and Fructus sojae (Fig. 3 and Fig. 4 ); Whereas the n-Butyl Ethanol Extract group and the Methylene Chloride Extract Group induced the cardiovascular toxicity of zebrafish (Figure 1 and Figure 2) and liver toxicity (Figures 3 and 4).