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目的 EBV作为肿瘤相关病毒已获公认 ,为探讨EBV与HCC的关系及与肝炎病毒有无协同作用 ,进行本研究。方法 采用PCP、RT -PCR及免疫组化方法检测石蜡包埋HCC标本中EBV、HBV、HCV和HDV。结果 PCR测HBVDNA(X基因和 (或 )S基因 )阳性率为 5 6 .4 % (4 4例 /78例 ) ,EBVDNA(BaimHⅠW和 (或 )LMP1)阳性率为 2 8.2 % (2 2例 /78例 )。RT -PCR测HCVRNA阳性率为 5 .6 % (1例 /18例 ) ,HDVRNA阳性率为 0 (0例 /18例 )。免疫组化测EBVLMPl多定位于肿瘤细胞中。结论 EBV在HCC组织中有较高的检出率 ,值得注意 ;HBV对HCC发生起重要作用 ,与EBV无明显关系 ;本地区HCV发病率不高 ,但其与EBV在HCC发生中有无协同作用值得进一步研究。HDV与HCC无明显关系。
The purpose of EBV as a tumor-associated virus has been recognized, in order to explore the relationship between EBV and HCC and hepatitis A virus with or without synergy, the study. Methods PCV, RT-PCR and immunohistochemistry were used to detect EBV, HBV, HCV and HDV in paraffin-embedded HCC specimens. Results The positive rate of HBVDNA (X gene and / or S gene) was 56.4% (44 cases / 78 cases) by PCR, and the positive rate of EBVDNA (BaimHIW and / or LMP1) was 22.2% (22 cases / 78 cases). The positive rate of HCV RNA in RT-PCR was 5.6% (1 case / 18 cases), and the positive rate of HDV RNA was 0 (0 cases / 18 cases). Immunohistochemical detection of EBVLMP1 in tumor cells. Conclusions EBV has a higher detection rate in HCC tissues, which is worth noting; HBV plays an important role in the occurrence of HCC and has no obvious relationship with EBV; the incidence of HCV in this area is not high, but is there any synergism with EBV in the development of HCC Role worth further study. HDV and HCC no significant relationship.