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以异硫氰酸胍裂解血清释放核酸使被二氧化硅吸附，经乙醇洗涤后用水悬浮，上清即含纯化ＤＮＡ。结合双阶变温ＰＣＲ建立一种简单快速的血清ＨＢＶ－ＤＮＡ检测方法。该法只需检测１０ｕｌ血清，完成提取和扩增只要３ｈ．操作很易掌握。用之检测５６例ＨＢｓＡｇ＋／ＨＢｅＡｇ＋的血清、４２例ＨＢｓＡｇ＋／ＨＢｅＡｇ－的血清和５４例ＨＢｓＡｇ／ＨＢｅＡｇ的血清，结果ＨＢＶ－ＤＮＡ检出率分别为８６％、５７％和１３％。应用表明这是一种简捷有效的核酸纯化和扩增方法，适于对大量临床样本作检测之用。
The guanidine isothiocyanate cleavage of serum release of nucleic acid adsorption by silica, washed with ethanol and suspended in water, the supernatant containing purified DNA. A simple and rapid serum HBV-DNA detection method was established by using two-step PCR. The method only detects 10ul serum, complete extraction and amplification as long as 3h. Operation is easy to grasp. Fifty-six serum samples of HBsAg + / HBeAg +, 42 serum samples of HBsAg + / HBeAg- and 54 serum samples of HBsAg / HBeAg were detected. The detection rates of HBV-DNA were 86%, 57% and 13% respectively. Application shows that this is a simple and effective nucleic acid purification and amplification method, suitable for a large number of clinical samples for testing.