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耐低氮种质的发掘及其在高原粳稻中应用,有利于提升高原稻区的生产效率并保护环境。在云南高原稻区两种土壤氮素水平下,种植以育成的优质高原粳稻品种滇粳优1号(DJY1)为轮回亲本所形成的2064份导入系,以叶绿素计读数(SPAD)值为指标,评价导入系的耐低氮特性。结果显示:供试导入系的SPAD平均值在两种不同土壤氮素水平下均呈正态分布,且与有效穗和穗粒数极显著正相关;以超出95%右尾概率值为标准,初步筛选出供体亲本为Khazar(BC3F3)和Chhomrong(BC3F4)2个导入系为低氮钝感材料,即耐低氮材料;以小于5%左尾概率值为标准,则推论出供体亲本为Basmati370(BC3F3)、Type3(BC3F3)、Khole-Mavshi(BC3F3)、Ajaya(BC3F3)、Doddi(BC3F3)和鱼秋谷(BC4F3)的6个导入系为低氮敏感材料。这些材料可用于高原稻区耐低氮及氮高效育种及遗传研究。
The discovery of low-nitrogen-resistant germplasm and its application in plateau japonica rice are favorable for improving the production efficiency and protecting the environment in the plateau rice area. A total of 2064 lines of inbred lines were selected from the japonica rice cultivar Dianyinggyou 1 (DJY1) which was bred in the Yunnan Plateau under the two soil nitrogen levels in the Yunnan plateau. Chlorophyll meter readings (SPAD) were used as indexes To evaluate the low-nitrogen-tolerant properties of the introduction lines. The results showed that the average value of SPAD in the introduced lines was normal distribution at two different soil nitrogen levels, and was significantly and positively correlated with the effective spikes and grains per spike. With the right-tail probability value exceeding 95% as the standard, Two donor lines, Khazar (BC3F3) and Chhomrong (BC3F4), were selected as low nitrogen insensitive materials, ie, low nitrogen tolerant materials. The results showed that the donor parents Six of the six inbred lines for Basmati370 (BC3F3), Type3 (BC3F3), Khole-Mavshi (BC3F3), Ajaya (BC3F3), Doddi (BC3F3) and Yakutani (BC4F3) were low nitrogen sensitive materials. These materials can be used for high-low-nitrogen and nitrogen-resistant breeding and genetic research in plateau areas.