目的探讨山东老年人载脂蛋白E(apoE)基因表型及频率分布。方法采用等电聚焦电泳及免疫印迹法测定2102例健康老年人apoE表型及频率分布。结果山东省老年人群中存在6种基因型,其中以E3/3为主,占总基因频率的66.0%;人群中ε2、ε3、ε4频率分别为4.4%、82.7%和12.9%,男女基因频率差异无统计学意义(P>0.05)。结论山东老年人群中apoE表型以E3/3最常见,apoE等位基因频率以ε3最高。 Objective To investigate the phenotype and frequency distribution of apoE gene in Shandong elderly. Methods The apoE phenotypes and frequency distribution of 2102 elderly healthy people were determined by isoelectric focusing electrophoresis and Western blotting. Results There were 6 genotypes in the elderly population in Shandong Province, of which E3 / 3 was the major gene, accounting for 66.0% of the total. The frequencies of ε2, ε3 and ε4 in the population were 4.4%, 82.7% and 12.9% respectively. The difference was not statistically significant (P> 0.05). Conclusion The apoE phenotype of Shandong elderly population is the most common E3 / 3, the apoE allele frequency is the highest ε3.