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以辣椒下胚轴为外植体,通过农杆菌介导将 CMV 和 TMV 外壳蛋白基因导入线辣椒,获得了转基因线辣椒。在温室人工接种 CMV 和 TMV 后,优选出高抗 CMV 和 TMV 的纯合系。PCR、Southern blot、RT-PCR、Western blot 分析证明纯台系 T3代植株的遗传稳定性。纯合系16-13株系的 T3、T4代植株环境释放试验表明其抗病性和农艺性状优良,产量是对照组的1.48-2.10倍。按国家标准对干果的品质进行了分析,各项指标与对照组相当。2000年转基因辣椒干果制品依据《食品安全性毒理学评价程序和方法》(GB15193-94)进行了急性经口毒性试验、三十天喂养试验、微核试验、精子畸型试验和 Ames 试验。检验结
The hypocotyls of pepper were used as explants and the CMV and TMV coat protein genes were introduced into the capsicum via Agrobacterium tumefaciens to obtain transgenic lines of pepper. After artificial inoculation of CMV and TMV in the greenhouse, homozygous lines highly resistant to CMV and TMV are preferred. PCR, Southern blot, RT-PCR and Western blot analysis proved the genetic stability of T3 generation of pure Tai line. The environmental release test of T3 and T4 plants of homozygous line 16-13 showed that their disease resistance and agronomic traits were excellent, and the yield was 1.48-2.10 times that of the control group. The quality of dried fruits was analyzed according to the national standards, and the indexes were comparable with those of the control group. In 2000, the genetically modified pepper dried fruit products were subjected to acute oral toxicity test, thirty day feeding test, micronucleus test, sperm abnormality test and Ames test according to “Food Safety Toxicology Evaluation Program and Method” (GB15193-94). Check knot