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Lung cancer is a common type of cancer that causes a very large public health burden world-wide.Achieving a better understanding of the molecular mechanism underlying the progression oflung cancer is of benefit for the diagnosis,prognosis,and treatment of lung cancer.Here,we first identified dramatically decreased expression of miR-338-5p in lung cancer tissues and cells using quantitative polymerase chain reaction(qPCR)analysis.We then revealed that miR-338-5p inhib-ited the cell growth and migration of lung cancer cells using cell counting kit 8(CCK8),EdU,and Transwell analysis.Furthermore,we demonstrated that miR-338-5p inhibited METTL3 expression by qPCR,western blot analysis,and luciferase reporter assay,while upregulation of METTL3 alle-viated the role of miR-338-5p in lung cancer cells.We also showed that METTL3 promoted c-Myc expression by increasing the m6A modification of c-Myc,and overexpression of c-Myc restored the inhibition of cell growth and migration of lung cancer cells induced by METTL3 silencing.Ultimately,this research illustrated that modification of the miR-338-5p/METTL3/c-Myc pathway affected cellular progression in lung cancer cells.Collectively,our study revealed the underlying mechanism of miR-338-5p in lung cancer,providing a novel regulatory pathway in lung cancer.There is potential for this pathway to serve as a diagnostic,prognostic,and therapeutic biomarker for lung cancer.