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目的探讨半乳糖凝集素-3(Gal-3)在小鼠柯萨奇病毒B3(CVB3)所致病毒性心肌炎(VMC)中的表达及其临床意义。方法选取58只健康雄性Balb/C小鼠,随机分为VMC组(n=50)和对照组(n=8)。VMC组小鼠腹腔接种半数组织培养感染剂量(TCID50)为10-5L-1的Nancy株CVB3病毒液0.1 mL,分别于接种第7、10、14、28天随机取8只处死;对照组小鼠腹腔接种不含病毒的eagle氏液0.1 mL,于第28天处死。光镜下观察小鼠心肌组织的病理变化并计算心肌病理积分;运用实时荧光定量PCR检测心肌组织中Gal-3 mRNA的表达,应用ELISA法检测外周血Gal-3水平,并与心肌病理积分作直线相关性分析。结果 1.与对照组小鼠比较,VMC组小鼠心肌组织Gal-3 mRNA的表达在第7天(0.91±0.64 vs 10.09±2.78)、第10天(0.91±0.64 vs 12.49±6.08)和第14天(0.91±0.64 vs 2.86±2.90)均明显增高(Pa<0.05)。2.与对照组小鼠外周Gal-3水平(5.27±1.11)比较,VMC组小鼠外周血Gal-3水平在第7天(9.70±2.39)、第10天(8.98±3.03)和第14天(7.73±1.88)也均明显增高(Pa<0.05)。3.小鼠心肌组织中Gal-3 mRNA与外周血中Gal-3表达水平均与心肌病理积分呈直线正相关(r=0.77、0.69,Pa<0.05)。结论 Gal-3参与VMC的发生与发展,且与VMC的病情严重程度有关,可作为VMC新的检测指标。
Objective To investigate the expression of galectin-3 (Gal-3) in viral myocarditis (VMC) induced by Coxsackievirus B3 (CVB3) in mice and its clinical significance. Methods Fifty healthy male Balb / C mice were randomly divided into VMC group (n = 50) and control group (n = 8). The mice in VMC group were inoculated intraperitoneally with 0.1 mL of CVB3 virus solution of Nancy strain with TCID50 of 10-5L-1 and were sacrificed at 7, 10, 14 and 28 days after inoculation. The control group Rats were intraperitoneally inoculated with 0.1 mL of virus-free eagle’s solution and sacrificed on the 28th day. The pathological changes of myocardial tissue were observed under light microscope and the myocardial pathological scores were calculated. The expression of Gal-3 mRNA in myocardial tissue was detected by real-time fluorescence quantitative PCR. The level of Gal-3 in peripheral blood was detected by ELISA. Straight line correlation analysis. Compared with the control group, the expression of Gal-3 mRNA in the myocardium of VMC group was higher on the 7th day (0.91 ± 0.64 vs 10.09 ± 2.78), the 10th day (0.91 ± 0.64 vs 12.49 ± 6.08) and the 14 days (0.91 ± 0.64 vs 2.86 ± 2.90) were significantly higher (Pa than 0.05). The level of Gal-3 in the VMC group was significantly higher than that in the control group (5.27 ± 1.11) on the 7th day (9.70 ± 2.39), the 10th day (8.98 ± 3.03) and the 14th Day (7.73 ± 1.88) were also significantly higher (Pa than 0.05). Gal-3 mRNA in myocardium and the expression of Gal-3 in peripheral blood were positively correlated with myocardial pathological scores (r = 0.77,0.69, Pa <0.05). Conclusions Gal-3 is involved in the occurrence and development of VMC, and is related to the severity of VMC. It may serve as a new detection index for VMC.